GPD2 (glycerol-3-phosphate dehydrogenase)

glycerol-phosphate shuttle

Alzheimer disease

cancer stem cell

FoF1-ATP synthase

energy metabolism

2014
学長裁量優秀若手研究者奨励賞, 単一細胞のミトコンドリアエネルギー代謝測定系の開発と展開, 東京医科歯科大学

2011
若手奨励賞, Screening of ATP-synthesis regulatory factors in human mitochondria by MASC assay, 日本生体エネルギー研究会

Role of Kynurenine and Its Derivatives in the Neuroimmune System.
Makoto Fujikawa; Masashi Ueda; Kenta Maruyama
International journal of molecular sciences, Jun. 2024
25(13), 10.3390/ijms25137144

GPD2: The relationship with cancer and neural stemness.
Maimaiti Mikeli; Makoto Fujikawa; Tsutomu Tanabe
Cells & development, Dec. 2022
173, 203824, 203824, 10.1016/j.cdev.2022.203824

Identification of chemical compounds as an inhibitor of mitochondrial ATP synthesis, leading to an increased stress resistance and an extended lifespan in C. elegans.
Takako Ikeda; Jun-Ichi Kishikawa; Yuki Hayashida; Makoto Fujikawa; Ken Yokoyama
Biochimica et biophysica acta. Bioenergetics, Jul. 2020, Reviewed, No invitation
148281, 148281, 10.1016/j.bbabio.2020.148281

Contribution of GPD2/mGPDH to an alternative respiratory chain of the mitochondrial energy metabolism and the stemness in CD133‐positive HuH‐7 cells
Maimaiti Mikeli; Makoto Fujikawa; Kai Nagahisa; Shuhei Yasuda; Natsuhiko Yamada; Tsutomu Tanabe
Genes to Cells, Feb. 2020, Reviewed, No invitation
25(2), 139, 148, 10.1111/gtc.12744

General anesthetics cause mitochondrial dysfunction and reduction of intracellular ATP levels
Jun-ichi Kishikawa; Yuki Inoue; Makoto Fujikawa; Kenji Nishimura; Atsuko Nakanishi; Tsutomu Tanabe; Hiromi Imamura; Ken Yokoyama
PLoS ONE, Jan. 2018, Not reviewed, No invitation
13(1), e0190213, 10.1371/journal.pone.0190213

ATP合成活性を阻害して寿命伸長をもたらす物質の探索
林田 優希; 岸川 淳一; 藤川 誠; 今村 博臣; 横山 謙
生命科学系学会合同年次大会, Dec. 2017, Not reviewed, No invitation
2017年度, [2P, 0255]

ミトコンドリアのATP合成に対する新規阻害剤を発見するためのMASCアッセイを用いた化学物質スクリーニング(Screening of chemical compounds to find new inhibitors against ATP synthesis in mitochondria by MASC assay)
Hayashida Yuki; Kishikawa Jun-ichi; Fujikawa Makoto; Imamura Hiromi; Yokoyama Ken
生物物理, Aug. 2017, Not reviewed, No invitation
57(Suppl.1-2), S301, S301

Assembly of human mitochondrial ATP synthase through two separate intermediates, F1-c-ring and b-e-g complex.
Makoto Fujikawa
FEBS letters, Aug. 2015, Reviewed, No invitation
10.1016/j.febslet.2015.08.006

Population of ATP synthase molecules in mitochondria is limited by available 6.8-kDa proteolipid protein (MLQ)
Makoto Fujikawa; Shigenori Ohsakaya; Kanako Sugawara; Masasuke Yoshida
GENES TO CELLS, Feb. 2014, Reviewed, No invitation
Lead, 19(2), 153, 160, 10.1111/gtc.12121

Evaluation of intramitochondrial ATP levels identifies G0/G1 switch gene 2 as a positive regulator of oxidative phosphorylation.
Makoto Fujikawa
Proceedings of the National Academy of Sciences of the United States of America, Dec. 2013, Reviewed, No invitation
111(1), 273, 278, 10.1073/pnas.1318547111

Screening of protein kinase inhibitors and knockdown experiments identified four kinases that affect mitochondrial ATP synthesis activity
Kanako Sugawara; Makoto Fujikawa; Masasuke Yoshida
FEBS LETTERS, Nov. 2013, Reviewed, No invitation
587(23), 3843, 3847, 10.1016/j.febslet.2013.10.012

IF1, a natural inhibitor of mitochondrial ATP synthase, is not essential for the normal growth and breeding of mice.
Makoto Fujikawa
Bioscience reports, Sep. 2013, Reviewed
33(5), e00067, 10.1042/bsr20130078

Distribution and Dynamics of Intracellular ATP Level and their Regulation
IMAMURA Hiromi; ANDO Tomomi; FUJIKAWA Makoto
Seibutsu Butsuri, Jan. 2013
53(1), 20, 23, 10.2142/biophys.53.020

Implication of bidirectional promoters containing duplicated GGAA motifs of mitochondrial function-associated genes
Uchiumi F; Fujikawa M; Miyazaki S; Tanuma S
AIMS Molecular Science, 2013
1(1), 1, 26

Assessing actual contribution of IF1, inhibitor of mitochondrial FoF1, to ATP homeostasis, cell growth, mitochondrial morphology, and cell viability.
Makoto Fujikawa
The Journal of biological chemistry, Apr. 2012, Reviewed, No invitation
287(22), 18781, 18787, 10.1074/jbc.m112.345793

Effects of Thujaplicins on the Promoter Activities of the Human SIRT1 and Telomere Maintenance Factor Encoding Genes
Uchiumi F; Tachibana H; Abe H; Yoshimori A; Kamiya T; Fujikawa M; Larsen S; Honma A; Ebizuka S; Tanuma S
Pharmaceutica Analytica Acta, 2012
3(5), 1000159

MRT letter: Expression of ATP sensor protein in Caenorhabditis elegans.
Makoto Fujikawa
Microscopy research and technique, Oct. 2011, Reviewed, No invitation
75(1), 15, 19, 10.1002/jemt.21103

Turnover of ATP synthase subunits in F1-depleted HeLa and yeast cells.
Makoto Fujikawa
FEBS letters, Jul. 2011, Reviewed
585(16), 2582, 2586, 10.1016/j.febslet.2011.07.011

Knockdown of DAPIT (diabetes-associated protein in insulin-sensitive tissue) results in loss of ATP synthase in mitochondria.
Makoto Fujikawa
The Journal of biological chemistry, Feb. 2011, Reviewed
286(23), 20292, 20296, 10.1074/jbc.m110.198523

A sensitive, simple assay of mitochondrial ATP synthesis of cultured mammalian cells suitable for high-throughput analysis.
Makoto Fujikawa
Biochemical and biophysical research communications, Sep. 2010, Reviewed
Lead, 401(4), 538, 543, 10.1016/j.bbrc.2010.09.089

ATP concentration change in Caenorhabditis elegans
Jun-ichi Kishikawa; Makoto Fujikawa; Hiromi Imamura; Kayo Yasuda; Naoaki Ishii; Shohei Mitani; Hiroyuki Noji; Ken Yokoyama
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, Jul. 2010, Reviewed, No invitation
1797, 60, 61, 10.1016/j.bbabio.2010.04.197

ESE-3, an Ets family transcription factor, is up-regulated in cellular senescence.
Makoto Fujikawa
Cancer science, Jul. 2007, Reviewed
Lead, 98(9), 1468, 1475, 10.1111/j.1349-7006.2007.00543.x

Interaction of TIP26 from a hyperthermophilic archaeon with TFB/TBP/DNA ternary complex.
Makoto Fujikawa
Extremophiles : life under extreme conditions, Jun. 2001, Reviewed
5(3), 177, 182, 10.1007/s007920100193

波紋疾走 ほとばしる生命エネルギー
藤川誠
日本生物工学会, 2014
Corresponding, 92(4), 191, 191

低酸素により誘導されるミトコンドリアタンパク質MENTはATP合成を促進し低酸素から心臓を保護する
木岡 秀隆; 加藤 久和; 藤川 誠; 鈴木 俊治; 今村 博臣; 野地 博行; 吉田 賢右; 北風 政史; 朝野 仁裕; 小室 一成; 高島 成二
日本臨床分子医学会学術総会プログラム・抄録集, Apr. 2013, Not reviewed, No invitation
50回, 53, 53

Regulatory mechanism of ATP synthase in mitochondria
藤川誠; 横山謙
実験医学, 2009, Not reviewed
Lead, 27(7), 997, 1003

グリセロール–リン酸シャトルを担う酵素はがんと神経細胞の幹細胞 性に関与している
藤川誠; Mikeli Maimaiti; 田邊勉
第48回 日本生体エネルギー研究会, Dec. 2022, No invitation
Oral presentation

GPD1/2 共役によるグリセロールリン酸シャトルががん幹細胞性に寄与している
藤川誠; 安田柊平; Mikeli Maimaiti; 田邊勉
第46回 日本生体エネルギー研究会, 2020
Oral presentation

呼吸鎖XとしてのGPD2/mGPDHがCD133陽性HuH-7細胞のがん幹細胞性を支えている
藤川誠; ミヒライ マイマイティ; 安田柊平; 田邊勉
第45回日本生体エネルギー研究会, Dec. 2019, No invitation
Domestic conference

呼吸鎖XとしてのGPD2/mGPDHがCD133陽性HuH-7細胞のがん幹細胞性を支えている
藤川 誠; Maimaiti Mikeli; 安田 柊平; 田邊 勉
第42回日本分子生物学会年, Dec. 2019, No invitation
Poster presentation, Domestic conference

The glycerol-3-phosphate dehydrogenase activity in Cancer Stem Cells
Mikeli Maimaiti; Makoto Fujikawa; Tsutomu Tanabe
第44回日本生体エネルギー研究会, Dec. 2018, No invitation
Poster presentation, 千葉大学西千葉キャンパス, 千葉大学西千葉キャンパス, Domestic conference

がん幹細胞のエネルギー代謝
藤川誠; ミヒライ マイマイティ; 田邊勉
第44回日本生体エネルギー研究会, Dec. 2018, No invitation
Oral presentation, 千葉大学西千葉キャンパス, 千葉大学西千葉キャンパス, Domestic conference

哺乳類シングルセルのATP合成活性測定を目指して~iMASC (improved MASC) アッセイ法の開発~
藤川誠; 吉田賢右; 田邊勉
第41回 日本生体エネルギー研究会, 2015
Oral presentation

Novel factors essential for human mitochondrial FoF1-ATP synthase activity found by MASC screening
Fujikawa M
Tokyo ATPase Workshop, Jun. 2014, Invited
Poster presentation

ヒト FoF1 の d-サブユニット ノックダウン株を用いた FoF1 アセンブリ機構の解析
Fujikawa M; Sugawara K; Yoshida M
第38回 日本生体エネルギー研究会, 2013
Oral presentation

Functional identification of unknown genes localized at mitochondria using MASC assay
Fujikawa M; Mori M; Sugawara K; Yoshida M
日本細胞生物学会, May 2012
Poster presentation

IF1 (mitochondrial FoF1 inhibitor protein); from single molecule to knock-out mouse
T. Suzuki; M. Fujikawa; J. Nakamura; M. Yoshida
European Bioenergetics Conference, 2012
Oral presentation

Novel factors essential for human mitochondrial FoF1-ATP synthase activity found by MASC (Mitochondrial Activity of SLO-permeabilized Cells) screening
Fujikawa M; Yoshida M
European Bioenergetic Conference, 2012
Poster presentation

A novel mitochondrial protein m62, which is identified by MASC assay screening, is essential for FoF1-ATP synthase activity and the mRNA level is regulated by the amount of glucose supply
Fujikawa M; Yoshida M
日本分子生物学会, 2012
Poster presentation

Functional identification of unknown genes localized at mitochondria using MASC assay
Fujikawa M; Mori M; Yoshida M
日本分子生物学会, Dec. 2011
Oral presentation

Screening of ATP-synthesis regulatory factors in human mitochondria by MASC assay
Fujikawa M; Mori M; Yoshida M
第36回 日本生体エネルギー研究会, 2011
Oral presentation

Functional analyses of the minor subunits in FoF1 complex with a novel assay for mammal FoF1-ATP synthase
Fujikawa M; Mori M; Yoshida M
日本細胞生物学会, May 2010
Oral presentation

IF1 influences on energy metabolism not only under energy restricted condition but also under normal condition
Fujikawa M; Mori M; Yoshida M
European Bioenergetics Conference, 2010
Poster presentation

IF1 influences on energy metabolism not only under energy restricted condition but also under normal condition
Fujikawa M; Mori M; Yoshida M
日本分子生物学会, 2010
Poster presentation

Global analyses of human mitochondrial ATP synthesis regulation mechanism by MASC assay
Fujikawa M; Mori M; Yoshida M
第35回 日本生体エネルギー研究会, 2010
Oral presentation

A new assay for mitochondrial ATP synthesis of culture cells
Fujikawa M; Mori M; Yoshida M
日本分子生物学会, Dec. 2009
Poster presentation

A novel method for ATP synthesis activity assay of human FoF1-ATP synthase: It’s applicable to high throughput screening
Fujikawa M; Mori M; Yoshida M
第34回 日本生体エネルギー研究会, Dec. 2009
Oral presentation

IF1, an inhibitor protein of FoF1-ATP synthase, helps to maintain ATP level in human culture cells when ATP synthesis is troubled
Fujikawa M; Mori M; Suzuki T; Yoshida M
日本細胞生物学会, Jun. 2008
Poster presentation

ランダムシーケンス解析法による石油分解／合成細菌HD－1株に特徴的な代謝経路の探索
藤川 誠; 春木 満; 今中 忠行; 森川 正章; 金谷 茂則
日本生物工学会, Aug. 2000
Oral presentation

糖尿病とアルツハイマー病の血液エネルギー代謝 —予防と先制医療のための早期診断を目指して—
東京医科歯科大学
Oct. 2018 - Sep. 2019
花王健康科学研究会, 花王健康科学研究会, Principal investigator

膵島β細胞のインスリン分泌におけるミオシン軽鎖キナーゼの機能解析
基盤研究(C)
2017 - 2019
藤川 誠; 藤川 誠, 藤川 誠; 藤川 誠, 文部科学省/日本学術振興会, 文部科学省/日本学術振興会, Principal investigator

Creation of novel anticancer lead compounds targeting receptor-type tyrosine kinase Met molecule
Grant-in-Aid for Scientific Research (C)
Tokyo University of Science
2013 - 2015
田沼 靖一; 高澤 涼子; 藤川 誠, TANUMA Sei-ichi; TAKASAWA Ryoko, 文部科学省/日本学術振興会, Japan Society for the Promotion of Science, Coinvestigator

MASCアッセイ法を用いたエネルギー代謝関連遺伝子の機能同定
若手研究(B)
2012 - 2014
藤川 誠; 藤川 誠, 藤川 誠; 藤川 誠, 文部科学省/日本学術振興会, 文部科学省/日本学術振興会, Principal investigator

血球エネルギー代謝解析による認知症早期診断の開発研究
基盤研究(C)
藤川 誠; 藤川 誠, 藤川 誠; 藤川 誠, 文部科学省/日本学術振興会, 文部科学省/日本学術振興会

特開2011-205935, 特願2010-075564, ミトコンドリアの代謝活性測定方法
藤川誠, 吉田賢右